Product Info Summary
SKU: | A01053-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Doublecortin/DCX Antibody Picoband™
View all Doublecortin Antibodies
SKU/Catalog Number
A01053-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Doublecortin/DCX Antibody Picoband™ catalog # A01053-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Doublecortin/DCX Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01053-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Doublecortin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01053-1 is reactive to DCX in Human, Mouse, Rat
Applications
A01053-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
40.574kDa
Background of Doublecortin
Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X, is a protein that in humans is encoded by the DCX gene. This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Doublecortin using anti-Doublecortin antibody (A01053-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat brain tissue lysates,
lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Doublecortin antigen affinity purified polyclonal antibody (Catalog # A01053-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Doublecortin at approximately 40KD. The expected band size for Doublecortin is at 40KD.
Protein Target Info & Infographic
Gene/Protein Information For DCX (Source: Uniprot.org, NCBI)
Gene Name
DCX
Full Name
Neuronal migration protein doublecortin
Weight
40.574kDa
Alternative Names
DBCN; Doublecortex; Doublecortin; Doublin; FLJ51296; Lissencephalin-X; LISX; Lis-X; X-linked (doublecortin) DCX DBCN, DC, LISX, SCLH, XLIS doublecortin neuronal migration protein doublecortin|doublecortex|doublin|lis-X|lissencephalin-X
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCX, check out the DCX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Doublecortin/DCX Antibody Picoband™ (A01053-1)
Hello CJ!
A01053-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Xm, Du F, Yang D, Wang R, Yu Cj, Huang Xn, Hu Hy, Liu W, Fu J. Bmc Neurosci. 2011 Jun 24;12:61. Doi: 10.1186/1471-2202-12-61. Granulocyte Colony-Stimulating Factor Increases The Therapeutic Efficacy Of Bone Marrow Mononuclear Cell Transplant...
Zhang Xm, Du F, Yang D, Yu Cj, Huang Xn, Liu W, Fu J. Bmc Neurosci. 2010 Oct 25;11:138. Doi: 10.1186/1471-2202-11-138. Transplanted Bone Marrow Stem Cells Relocate To Infarct Penumbra And Co-Express Endogenous Proliferative And Immature Neuronal M...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
16 Customer Q&As for Anti-Doublecortin/DCX Antibody Picoband™
Question
I am interested in using your anti-Doublecortin/DCX antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We appreciate your inquiry. This A01053-1 anti-Doublecortin/DCX antibody is validated on mouse brain. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-30
Question
Would A01053-1 anti-Doublecortin/DCX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
You can see on the product datasheet, A01053-1 anti-Doublecortin/DCX antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-26
Question
My colleagues were well pleased with the WB result of your anti-Doublecortin/DCX antibody. However we have observed positive staining in frontal cortex cytoplasm. cell projection, using this antibody. Is that expected? Could you tell me where is DCX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
From what I have seen in literature, frontal cortex does express DCX. Generally DCX expresses in cytoplasm. cell projection,. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2019-07-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Doublecortin/DCX antibody A01053-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-03
Question
Is this A01053-1 anti-Doublecortin/DCX antibody reactive to the isotypes of DCX?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
The immunogen of A01053-1 anti-Doublecortin/DCX antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Doublecortin (74-100aa QSLRFHQNMELDFGHFDERDKTSRNMR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-09
Question
Would anti-Doublecortin/DCX antibody A01053-1 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of cervix carcinoma, DCX is highly expressed in cervix carcinoma. So, it is likely that anti-Doublecortin/DCX antibody A01053-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-11-14
Question
We have been able to see staining in rat fetal brain. Any tips? Is anti-Doublecortin/DCX antibody supposed to stain fetal brain positively?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
From literature fetal brain does express DCX. From Uniprot.org, DCX is expressed in frontal cortex, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2018-10-04
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-Doublecortin/DCX antibody A01053-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-09
Question
We are currently using anti-Doublecortin/DCX antibody A01053-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The anti-Doublecortin/DCX antibody (A01053-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-12
Question
I see that the anti-Doublecortin/DCX antibody A01053-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-26
Question
Is there a BSA free version of anti-Doublecortin/DCX antibody A01053-1 available?
B. Krishna
Verified customer
Asked: 2018-05-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Doublecortin/DCX antibody A01053-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-15
Question
Would anti-Doublecortin/DCX antibody A01053-1 work on monkey WB with brain?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
Our lab technicians have not tested anti-Doublecortin/DCX antibody A01053-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Doublecortin/DCX antibody A01053-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-29
Question
My question regarding product A01053-1, anti-Doublecortin/DCX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Taylor
Verified customer
Asked: 2017-11-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01053-1 anti-Doublecortin/DCX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-24
Question
Is a blocking peptide available for product anti-Doublecortin/DCX antibody (A01053-1)?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
We do provide the blocking peptide for product anti-Doublecortin/DCX antibody (A01053-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-08
Question
We want to test anti-Doublecortin/DCX antibody A01053-1 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Doublecortin/DCX antibody A01053-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Thomas
Verified customer
Asked: 2017-07-24
Answer
The products we sell, including anti-Doublecortin/DCX antibody A01053-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-24
Question
I was wanting to use your anti-Doublecortin/DCX antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
A. Miller
Verified customer
Asked: 2017-03-22
Answer
As indicated on the product datasheet, A01053-1 anti-Doublecortin/DCX antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-22